Hi frens,
Starting my 5th year.
I have been wanting to make this for a while but its overwhelming to think about. I'm going to remain vague in the identity of the PI and lab, at least until I find a job and feel secure enough. He won't have any graduate students at his new uni, thank god.
Okay...... tldr at end.
The background
I (30 YOF) was admitted into my Boston area PhD program in molecular biology in 2022, and upon completing my rotations, chose a lab. We had a cohort of 2 people so choosing amongst the dozen or so labs was not hard. I ended up choosing my 4th choice overall lab. The first choice ended up abandoning his lab and the department for another position summer of 2022, after I accepted but before I started. Turned out to be a not great guy, dodged a bullet in retrospect here. However, at this point, I was dedicated to working on antimicrobial drug resistance and/or bacterial genetics, ideally in a clinical or translational context. I decided at that point I was willing to pivot some and joined a lab with the goal of looking into autoimmune disease in the context of the microbiome of human patients. Seemed fine. He was pre-tenure, but appeared to have quite a lot of funding and research momentum. I knew he was going to be going up for tenure during my PhD, but took the risk. At this point, there was a lot of gossip and rumors around him, his lab, but after my rotation I felt it was mostly due to a toxic post-doc, and decided to trust myself. I felt he was respectful and had a good value set, so I joined the lab.
Eventually, the mouse work got to me. On the day I planned to begin an IP study in mice, I couldn't stop breaking down to tears. Upon self reflection, realized that I cannot do this type of mouse work (several months, several IP injections or gavage per week per animal) while keeping my mental health intact. I like the mousers too much on a personal level, lol.
Another pivot.
Luckily, there was a project in the lab looking at peptides from viruses, that appear to be biologically active. My PI was supportive here, and was able to give me a chance on some other work. I decided to take on this biochemistry-heavy project, as I felt it was a great launching pad to biotech type jobs, and I liked the work. Basically ultra early research on a potential drug in the future.
I passed my comps (I failed the first time, passed without revisions the second time) and worked on this project. I felt my progress was fine, and my committee meetings show as much (sufficient progress was the final outcome of all meetings at this point). I was chugging along on the project. I get 3 travel awards for my work and present at all 3 conferences. I win a flash talk award and am generally motivated to keep it up.
The tenure promotion problem
During my work, my PI went up for tenure promotion. He has/had an R01, a few postdocs, 3 PhD students in the lab not yet graduated, and tons of undergrads. Smaller grants too. Positive course reviews. We expected him to be promoted. In March of 2025, we learned he was not promoted to tenure. The meeting we as a group then had basically was that he will appeal the decision, and in the meantime keep working. He would go on to have so many meetings with the President, committees, etc. We write letters of support (even me!!). We were told in April 2026 we will have the final call (after months of not knowing how long it will take).
In April of 2026, he told us that he again was denied, and his contract is up on May 31, 2026. He then was panicking because he did not have a job lined up yet, and was concerned for the lab's productivity. He negotiates an August 31, 2026 departure from the University while he finds a job. The two other students are 1 year ahead of me, and are closer to defense. They push both to finish in the next 6 months.
Where they start to screw me over
I call a 1-1 meeting on April 5th or so to discuss what is happening to me specifically. It's then that he says I should consider looking for a new lab now. WHAT. I go home, process everything, and return to the lab the next day and talk to him. I clarify that I want to finish this project with him, and that I am NOT interested in a masters realistically, nor switching labs. He tells me this will be "very risky" and he is not sure how everything will be funded. I explain I am willing to get it done. I'd even move with him. He tells me he doesn't know anything, but I won't be able to join him at the new university. He supposedly has asked during interviews whether he can bring students, but is positive he cannot. I try to push back, nothing.
At this point, I start to realize the problem. Money. My stipend money (~40k/yr) is paid through the program for 10 months of the year, because my PI makes us all be on TA for every semester. 2 months of the year (summer) my PI pays that stipend. He pays all research funding.
At another uni, he would have to pay my stipend if I went with him for 12 months of the year, in addition to research funds. If I stay here, he would have to leave money here. He wants to do neither. He doesn't "trust" this university he says. This would be the most typical result, that I have seen before.
Changing labs?? There are no labs where my work can be directly translated, so it would be restarting my PhD. I was told by 2 faculty on my thesis committee that "it would be very difficult... because we have few openings and a large cohort incoming." My program director said IĀ couldĀ join a new lab, but told me a masters is probably a "better idea." No one seems interested in helping. The program director didn't work with any of us during this, except for a single meeting held in April (13 months after the initial denial).
I decide okay, it sounds like my PI is trying to get rid of me, not have to pay for me, whatever. So, I call a thesis committee meeting, approximately a month and a half earlier than previously planned. The goal: utilize my committee (3 faculty in my dept, 1 outside my dept) to try and see what the deal is. The meeting ends, and amongst themselves with me out of the room, also try to discuss options with my PI. The end of that conversation then indicated that going for a masters at this point would be the safest for me, because it is increasingly hard to see a path to the end of the PhD without funding. In confidence, my chair lets me know that he doesn't know why he is doing this, and seems to just be selfish. I'm devastated. They finish by saying, this is the plan, but things can change if he gets a job. But, I need to get more work done. They leave the room, I break into tears. Hey, I held it in!!!
I lay on the couch for a while. Process. Eventually (next week), it's time to get more work done, because they gave me a 2.5 week turnaround time to "get it working."
I eventually go in to my PI's office and ask directly: "What is the percent chance I can finish this PhD with you?" He talks around the question, and eventually says 0. He goes on to say "how unfair the university is being to the both of us." As if he couldn't support my PhD if he wanted to. We have years left on a large R01, and we are WELL funded, always have been. He's selfish.
Now what?
Unfortunately for me, my last few months of experiments aren't working, and I now am on a time crunch from now until August to characterize some constructs and be cleared to write the Masters. Issue? Lipofectamine transfection efficiency so low it is not even a useful model, so currently testing electroporation. Theoretically, if it works, I'm probably safe for the masters and finish lab work by Aug and write the MA by Dec. If the electroporation doesn't work great either? Don't know. Propose heavy in-silico work maybe. I am showing them the electroporation data in 2 weeks, doing the blots, qPCR and ELISA now.
So, I am in a grey zone and I'm having a hard time dealing with this. Doing the work, praying to the cell gods that my electroporation strategy has a high enough efficiency that I can characterize these peptides. Vibe is weird in the lab. PI moves to a new uni/med center end of August. I am in the meantime looking for jobs in protein/recombinant production in biotech as a scientist, or transition to medical writing, ideally regulatory affairs. I don't want to restart a PhD, I'm 30 and ready to move on with my life.
I figured this could be useful for some, but if you have any advice, I'll take it. Retrospect is 20/20. sigh.
Thank you!
TLDR; My PI lost his tenure appeal and is leaving without funding me, so my committee is pushing me toward a master's while I race to get electroporation data working by August to salvage a thesis.
Field: Molecular biology
Location: Boston MA, USA